Course Title: Medical Mycology Course Code: CLS 473 Program: Clinical laboratory sciences Department: Clinical laboratory sciences College: Applied Medical sciences Institution: Taibah University Version: 2 Last Revision Date: 01/01/1438 AH A. General information about the course: 1. Course Identification 1. Credit hours: (3) 2 T + 1 P 2. Course type A. ? University ?College ? Department ?Track ?Others B. ? Required ?Elective 3. Level/year at which this course is offered: (level 7 /Year 4) 4. Course general Description: The course focuses on the following theoretical and practical components: - -The diagnosis (mycological, serological, immunological, and histopathological) identification of pathogenic fungi -The application of molecular techniques used in characterization of clinical fungal specimens -Toxicological aspects of pathogenic fungi on human body systems and various complications due to {fungal infections. -Knowledge in transmission, pathogenesis, treatment and prophylaxis of fungal infections. -Knowledge of the pathological interactions between fungi and other microbial groups bacteria, viruses, parasites) also. - Emerging Fungal Threats and the future of fungi 5. Pre-requirements for this course (if any): Medical Microbiology CLS 242 6. Co-requisites for this course (if any): N/A 7. Course Main Objective(s): The purpose of this course is to develop theoretical knowledge and enhance practical skills of students in the field of Medical Mycology. 2. Teaching mode (mark all that apply) No Mode of Instruction Contact Hours Percentage 1 Traditional classroom 48 80% 2 E-learning 12 20% 3 Hybrid • Traditional classroom • E-learning 4 Distance learning 3. Contact Hours(based on the academic semester) No Activity Contact Hours 152. Lectures 30 153. Laboratory/Studio 30 154. Field 155. Tutorial 156. Others (private study/learning hours expected for students per week) 0 Total 60 B. Course Learning Outcomes (CLOs), Teaching Strategies and Assessment Methods Code Course Learning Outcomes Code of CLOs aligned with program Teaching Strategies Assessment Methods 1.0 Knowledge and understanding 1.1 Describe mycological characteristics such as structure, classification, diseases and diagnosis K1 Continuous discussions with the -Exams, quiz, presentations, Homework. Code Course Learning Outcomes Code of CLOs aligned with program Teaching Strategies Assessment Methods 1.2 Demonstrate application of serological, histopathological, and molecular techniques for fungal detection, and illustrate toxicological effects of pathogenic fungi on human body systems and associated complications K2 students during the Lectures. -Lectures are designed to develop. the directions for extra reading by the students on the topics. - The students are instructed to prepare. topics from the text book - Power point presentations include. illustrative figures and pictures throughout -Theoretical Exams would contain: -Objective questions (Factual recall, illustrative and./or extended MCQs & fill in blanks). -Short essay questions 2.0 Skills 2.1 Apply safety methods and quality standards used in Mycology laboratory to ensure safety and produce reliable results S1 Lectures and class seminar -Internet searches for studying. selected topics. - Using the microscopes, slides, and other instruments freely and directly during the practical classes under supervision of the instructor. -Quizzes. -Objective Structured Practical Examination (osPE). -Problem solving questions -Group and individual evaluation of the internet search 2.2 Optimize sample selection for fungal pathogen identification and evaluate pathogenic fungi, correlating symptoms with characteristics, mycotoxins, and prophylaxis, through data analysis for evidence-based diagnosis and interpretation S Code Course Learning Outcomes Code of CLOs aligned with program Teaching Strategies Assessment Methods 3.0 Values, autonomy, and responsibility 3.1 Demonstrate presence of responsibility ethical behavior, pursuing professional growth, and the use of equipment and resources of the department in a responsible manner. V1 - Using the internet and library for searching scientific literature. -Encourage them to attend lectures. regularly. -helping them to catch up on missed. lectures. - Give them tasks individually and in groups outside the syllabus. -Discussions during the lectures. - Surprise tests on previous lectures, Assignments on some topics relevant to the subject but outside the syllabus. -Discussions during the lectures. 3.2 Demonstrate ability to work both independently and within groups sharing the learning facilities like the microscopes, autoclaves etc with his/her colleagues and record of results independently and professionally. V2 C. Course Content No List of Topics theory Contact Hours 58. Introduction to Medically important fungi: characteristic, morphology, structure, medical significance, serological, immunological and histopathological and molecular 2 59. Characteristics and diagnostic procedures and cultural differentiation between fungal, bacterial and viral diseases 2 3 Superficial fungal infection Serological, immunological, histopathological and molecular characteristics 2 4 Subcutaneous fungal infection 1: Serological, immunological, histopathological and molecular characteristics 2 5 Subcutaneous fungal infection 2: Chromoblastomycosis, and Sporotrichosis infection immunological and histopathological and molecular diagnostic procedures 2 6 Systemic fungal infection: aetiological agents immunological, histopathological, and molecular diagnostic procedures 2 7 True pathogenic infection: Histoplasmosis, Blastomycosis, Coccidioidomycosis, and Pneumocystis 2 8 Medically Important Opportunistic mould infection immunological, Histopathological, and molecular diagnostic procedures 2 9 Medically Important Opportunistic yeast infection immunological, Histopathological, and molecular diagnostic procedures 2 10 Mycotoxins and Miscellaneous mycological procedures for fungal pathogen 2 11 Antifungal: action, synergistic and antagonistic effect regarding bacterial antibiotics. 2 12 Antifungal resistance: mechanisms and detection 2 13 Fungal action, synergistic and antagonistic effect regarding bacterial antibiotics 2 14 Molecular Diagnostic Techniques generally used in disease diagnosis. 2 15 Emerging Fungal Threats and the future of fungi 2 # List of Practical theory Contact hours 1 General characters of human pathogenic fungi: isolation, culturing, subculturing 2 2 Microscopical examination from clinical specimens and cultures 2 3 Diagnosis of fungal infection in various infected clinical specimens 2 4 Diagnosis of human Candidiasis, Cryptococcosis and other yeast infections 2 5 Fungal Diagnosis of superficial (dermatophytes) fungal infection, immunological and pathological examination 2 6 Fungal, serological, and immunological diagnosis of medical fungal agents from various lesions 2 7 Examination of subcutaneous fungal infection: Diagnostic serological immunological, histopathological, detection of fungal antibodies in blood serum, serological and immunological, Histopathological diagnosis of the group 2 8 Pathological diagnosis of systemic fungal infection 2 9 Diagnosis of Opportunistic mould infection immunological, Histopathological, and molecular diagnostic procedures 2 10 Mycotoxin purification and Miscellaneous procedures for fungal pathogen 2 11 Effect of fungal pathogens on living cells and extraction of fungal toxins and its effect on living tissues 2 12 Pathological, Histopathological and Biochemical testing of fungal infection 2 13 Immunological & serological assays for fungal diagnosis 2 14 Molecular diagnosis of human pathogenic fungi (yeast) 2 15 Molecular diagnosis of human pathogenic fungi (mould) 2 Total 60 D. Students Assessment Activities No Assessment Activities * Assessment timing (in week no) Percentage of Total Assessment Score 102. Continuous assessment (quiz , lab-book) Weeks 1-10 5% 103. Midterm examination (written) Week 8-9 15% 104. Assignment submission or case study Week 12 5% 105. Lab Reports Week 14 5% 106. Practical exam Week 15-16 30% 107. Final written examination Week 17-18 40% *Assessment Activities (i.e., Written test, oral test, oral presentation, group project, essay, etc.). E. Learning Resources and Facilities 1. References and Learning Resources Essential References Required textbook: 1- Larone’s Medically Important Fungi by Thomas J. Walsh, Randall T. Hayden, Davise H. Larone 2- Medical Mycology, K. J. Kwon-Chung and John E. Bennett, 2010. 3- Human Pathogenic Fungi: Molecular Biology and Pathogenic Mechanisms, Derek, J.Sulivan and Gary P. Moran 2014. Essential references: 1- Medical Mycology - William G. Merz, Roderick J. hay - Hodder Arnold ASM press, 2010. 2- Medical Mycology, David Ellis, The University of Adelaide, 2009. 3- Atlas of Clinical Fungi, 2008, G. S. de Hoog, J. Guarro, J. Gené, M. J. Figueras. Supportive References Virtual lecture: https://medium.com/@reuelmbennett/mycology-online-classmoc-6287edddd575 https://www.adelaide.edu.au/mycology/node/786 Electronic Materials 1- http://www.life-worldwide.org/ 2- https://www.aspergillus.org.uk/ 3- https://drfungus.org/ 4- https://www.labster.com/facultyresources/?k=Taibahufaclty2020– 5- https://mycology.adelaide.edu.au/laboratory/