Course Title: Serological Techniques Course Code: CLS 360 Program: Clinical Laboratory Sciences Department: Clinical Laboratory Sciences College: Applied Medical Sciences Institution: Taibah University Version: V1 Last Revision Date: N/A A. General information about the course: 1. Course Identification 1. Credit hours: (3 CH) 1T + 1P 2. Course type A. ? University ? College ? Department ? Track ? Others B. ? Required ? Elective 3. Level/year at which this course is offered: Level: 6 Year: 3 4. Course general Description: This course introduces the basic and complex serological techniques used in clinical laboratories. The course aims to equip students with the necessary serological applications in the serology and immunology laboratories and their clinical significance and disease correlation. Topics include traditional techniques and molecular methods for detection and confirmation of disease states. 5. Pre-requirements for this course (if any): CLS 237 6. Co-requisites for this course (if any): None 7. Course Main Objective(s): • Explain the principles behind key tests such as ELISA, immunodiffusion, Western blot, fluorescence microscopy, immunochromatography, agglutination, hemolysis, flow cytometry and electrophoresis. • Describe quality assessment practices for serology. 2. Teaching mode (mark all that apply) No Mode of Instruction Contact Hours Percentage 1 Traditional classroom 45 100% 2 E-learning 0 0 3 Hybrid • Traditional classroom • E-learning 0 0 4 Distance learning 0 0 3. Contact Hours(based on the academic semester) No Activity Contact Hours 116. Lectures 15 117. Laboratory/Studio 30 118. Field 0 119. Tutorial 0 120. Others (specify) 0 Total 45 B. Course Learning Outcomes (CLOs), Teaching Strategies and Assessment Methods Co de Course Learning Outcomes Prog PLO Teaching Strategies Assessment Methods 1.0 Knowledge and understanding 1.1 Describe the antigen and antibody interaction. K1 1. Interactive Lectures 2. Group discussion • Examination (MCQs, Short answer questions and long answer questions) 1.2 Acquire comprehensive knowledge of different serological techniques used in clinical laboratories. K3 1. Interactive Lectures 2. Group discussion • Examination (MCQs, Short answer questions and long answer questions) 2.0 Skills 2.1 Demonstrate the ability to distinguish between the different principals of the diagnostic methods in immunology. S1 1. Interactive Lectures 2. Group discussion 3. Case studies 4. Formal and interactive Lectures 5. Practical sessions • Practical Exam • Quizzes • Assignments and reports • Student evaluation 2.2 Analyze clinical case, connecting theoretical understanding with practical application enhancing diagnostic skills and fostering critical thinking. S2 2.3 Evaluate viral and bacterial infections using serological techniques S3 3.0 Values, autonomy, and responsibility 3.1 Conduct independent learning skills by actively seeking out, analyzing, and applying relevant scientific literature to deepen understanding of serological techniques. V1 Research topic in serological techniques field • Research Paper Assessment rubric C. Course Content No List of Topics Contact Hours 37. Antigen and Antibody Structure 3 38. Agglutination and neutralization 3 39. Precipitation 3 4. Immunodiffusion and Electro-immunodiffusion 3 5. Immunoelectrophoresis 3 6. Complement fixation test 3 7. Immunofluorescence Assays 3 8. Enzyme Immunoassays 3 9. Radial Immunoassays 3 10. Flow Cytometry 3 11. Diagnostic Serology 1 (Widal test, VDRL test, RPR test, Khan test, Wassermann Complement Fixation Test, Malaria Antigen Test, Dengue Rapid Diagnostic Test) 3 12. Diagnostic Serology 2 (ASO Latex Agglutination Test, Rose Waaler Test, Malaria Antigen Test, Dengue Rapid Diagnostic Test) 3 13. Diagnostic Serology 3 (RF latex Agglutination test, ASO latex Agglutination test, C-reactive protein, Pregnancy Test) 3 14. Diagnostic Serology 4 (Anti-Nuclear Antibody Test, Blood Group Test, Fibrin D-Dimer Test, Fibrin/Fibrinogen Degradation Products Test) 3 15. Quality Control and Assurance for Serological Techniques 3 Total 45 D. Students Assessment Activities *Assessment Activities (i.e., Written test, oral test, oral presentation, group project, essay, etc.). No Assessment Activities * Assessment timing (in week no) Percentage of Total Assessment Score 75. Assignment submission Week 12 5% 76. Lab Reports Week 14 5 % 77. Continuous assessment All over the semester 5% 78. Midterm examination (written) Week 8-9 15% 79. Practical exam Week 15-16 30% 80. Final written examination Week 17-18 40% E. Learning Resources and Facilities 1. References and Learning Resources Essential References Turgeon ML. Immunology and Serology in Laboratory Medicine. 4th Edition, 2008. ISBN 978- 0323043823 Supportive References Alhabbab RY. Basic Serological Testing. 1st Edition, 2018. Electronic Materials NA Other Learning Materials NA 2. Required Facilities and equipment Items Resources Facilities Classrooms Technology equipment (projector, smart board, software) Data Show, Smart Board, Computers Other equipment (depending on the nature of the specialty) N/A F. Assessment of Course Quality Assessment Areas/Issues Assessor Assessment Methods Effectiveness of teaching Instructor ü Direct Method (Rubrics) ü Indirect Method (Questionnaire on the course by the end of the semester) Assessment Areas/Issues Assessor Assessment Methods Effectiveness of Students' assessment Head of the department Student achievement is verified by the head of the department. Quality of Learning resources Instructor Student ü Course Report. ü Course Evaluation Survey (CES) Achievement of Course Learning Outcomes Instructor Students ü Direct Method (Rubrics) ü Indirect Method (Questionnaire on the course by the end of the semester) ü Course Evaluation Survey (CES) Effectiveness of Assessment Results Instructor ü Course Report ü Grade Report Analysis ü Course Evaluation Survey (Indirect) Assessors(Students, Faculty, Program Leaders, Peer Reviewer, Others (specify) Assessment Methods(Direct, Indirect)