Course Title: Blood Bank Course Code: CLS 471 Program: Clinical Laboratory Sciences Department: Clinical Laboratory Sciences College: Applied Medical Sciences Institution: Taibah University Version: V2 Last Revision Date: 20 November 2016 A. General information about the course: 1. Course Identification 1. Credit hours: ( 3 ) 2 T + 1 P 2. Course type A. ? University ?College ? Department ?Track ?Others B. ? Required ?Elective 3. Level/year at which this course is offered: (Level 7/year 4) 4. Course general Description: This course provides an overview of blood banking, focusing on the collection, storage, and distribution of blood and its products. It covers transfusion principles, procedures, and clinical significance of test results. Topics include pre-transfusion testing, adverse effects, donor selection, blood components, and related laboratory techniques such as cross-matching and antibody identification. Emphasis is placed on quality control, safety protocols, and building confidence in transfusion medicine practice. Students will gain the knowledge and skills needed for careers in blood bank professions. 5. Pre-requirements for this course (if any): Hematology (2) CLS 355 6. Co-requisites for this course (if any): None 7. Course Main Objective(s): 1- Describe the characteristics of antigens located on red blood cells, white blood cells, and platelets. 2- Diagram the basic structure of an IgG molecule and label the following components: heavy and light chains, Fab, and Fc regions, variable region, hinge region, antigenbinding site, and macrophage-binding site. 3- Compare IgM and IgG antibodies with structure, function, and detection by agglutination reactions. 4- List several routine tests performed in the immunohematology laboratory. 5- Describe the basic principles of antiglobulin testing. 6- State the methods for evaluating a positive direct antiglobulin test. 7- Define major and minor blood group systems like ABO, Rhesus, Kell, Kid, etc.… with regard to serologic and genetic classifications. 8- Describe the types of hemolytic disease of the fetus and newborn, antibodyspecificity involved, testing to identify the specificity and treatment for each. 9- List the adverse complications of blood transfusion and state the cause, and if appropriate, treatment of each. 10- Describe the types of immune hemolytic anemias including results of serologic testingand compatibility testing which must be performed. 11- State the requirements for performing compatibility testing and providing transfusionsupport for organ transplants. 12- Describe the donor selection process and accurately determine donor eligibility whengiven results of donor screening tests. 13- Describe the preparation and indicate the appropriate use of blood components by determining the component needed based on laboratory data. 14- Describe the quality control which must be done including the specific tests, frequencyof testing. 2. Teaching mode (mark all that apply) No Mode of Instruction Contact Hours Percentage 1 Traditional classroom 60 100% 2 E-learning 3 Hybrid • Traditional classroom • E-learning 4 Distance learning 3. Contact Hours(based on the academic semester) No Activity Contact Hours 147. Lectures 30 148. Laboratory/Studio 30 149. Field 150. Tutorial 151. Others (specify) Total 60 B. Course Learning Outcomes (CLOs), Teaching Strategies and Assessment Methods Code Course Learning Outcomes Code Prog Teaching Strategies Assessment Methods 1.0 Knowledge and understanding 1.1 Recognize types of Ag-Ab reactions and factors affecting agglutination, and explaining donor selection criteria and blood donation types to determine eligibility K1 Interactive Lectures (Intended to provide a solid foundation of knowledge on each topic while also stimulating students' curiosity for additional reading and exploration) Group discussion (Faculty will encourage questions, discussion, and critical thinking during each lecture) Problem-based learning. Assignments - Written exams (MCQs, Short answers questions, Structural short note essays questions and Case studies, Long answer questions 1.2 Recognize major and minor blood group systems (ABO, Rhesus, Kell, etc.), including antigens, antibodies, immunoglobulin class, mode of stimulation, serological reactivity detection, and clinical significance, and discuss the etiology and characteristics of hemolytic disease of the fetus and newborn (HDFN) K2 1.3 Explain the method of preparation, storage conditions, shelf life, and quality control requirements for the blood products K3 2.0 Skills 2.1 Describe serological tests utilized in blood banks to assess transfusion reactions. Resolve crossmatch incompatibilities, discuss limitations, and interpret results accordingly S1 Practical laboratory sessions (hands-on experience will allow students to perform various blood bank tests to understand the practical implications of their theoretical knowledge) Case studies (will allow students to analyze lab result and provide accurate interpretation) Problem-based learning. Discussions during formal lectures and or seminars. Practical portfolio Lab reports designed to enable students to recognize, evaluate and challenge their own learning process. -Self-reflection is actively encouraged. - Practical exam - Objective Structured Practical Examinati on (OSPE) - Problem solving questions - Case Studies 2.2 Explain the purpose of prenatal tests in the evaluation of HDN, titers, and bilirubin analysis on amniotic fluid including type and screen, antibody identification, antibody Code Course Learning Outcomes Code Prog Teaching Strategies Assessment Methods 3.0 Values, autonomy, and responsibility 3.1 Demonstrate teamwork and respect for diverse perspectives recognizing the value of differing viewpoints in scientific progress. V3 C. Course Content No List of Topics Contact Hours 56. Antigen Antibody reactions in blood banking laboratory and factors affecting them and complement system 4 57. ABO blood group system and its discrepancies 4 3. Rhesus blood group system 4 4. Other blood group systems 4 5. Serological techniques used in blood banking -1 (Saline and additive techniques) 4 6. Serological techniques used in blood banking -2 (Commbs test) 4 7. Criteria for blood donation and types of blood donation 4 8. Compatibility testing and pre transfusion tests (1) 4 9. Cross matching 4 10. Hemolytic transfusion reactions and Non-hemolytic transfusion reactions 4 11. Blood products (1) 4 12. Blood products and Haempherasis (2) 4 13. Investigation of Transfusion Reaction 4 14. Hemolytic disease of newborn (ABO incompatibility and Rhesus incompatibility) 4 15. Quality control in blood bank 4 Total 60 D. Students Assessment Activities No Assessment Activities * Assessment timing (in week no) Percentage of Total Assessment Score 99. Assignment submission OR Case studies Week 12 5 % 100. Midterm examination (written) Week 8-9 15 % 101. Continuous assessment (Quizzes, Case studies, presentations, Discussion, Journal club, etc.) All around semester 5% 4. Lab Reports Week 14 5 % 5. Practical exam Week 15-16 30% 6. Final written examination Week 17-18 40% *Assessment Activities (i.e., Written test, oral test, oral presentation, group project, essay, etc.). E. Learning Resources and Facilities 1. References and Learning Resources Essential References 1- Modern Blood Banking & Transfusion Practices Seventh Edition by Denise M. Harmening 2-Immunohematology for Medical Laboratory Technicians (2010) 3-Technical Manual, 19th edition (Technical Manual of the American Associan of Blood Banks) (Technical Manual of the American Association of Blood Banks) 4-Dacie and Lewis Practical Haematology: Expert Consult: Online and Print by Barbara J. Bain FRACP FRCPath, Imelda Bates MB BS MD MA FRCPath, et al. | Oct 14, 2016 Supportive References Mollison's Blood Transfusion in Clinical Medicine, 12th Edition .Blackwell. Oxford,(2014) Electronic Materials http://www.bbguy.org/ https://www.labce.com/ http://www.aabb.org/developmen t/ education/Pages/default.aspx https://labtestsonline.org/lab/blo odbank/ https://www.labce.com/ Other Learning Materials NA 2. Required Facilities and equipment Items Resources facilities (Classrooms, laboratories, exhibition rooms, simulation rooms, etc.) Classrooms, laboratories Technology equipment (projector, smart board, software) Data Show, Smart Board, Computers Other equipment (depending on the nature of the specialty) Centrifuges ,ABO and Rhesus antisera ,Anti human globulins (Ig G and Ig M), consumables, access to samples and controls F. Assessment of Course Quality Assessment Areas/Issues Assessor Assessment Methods Effectiveness of teaching Instructor ü Direct Method (Rubrics) ü Indirect Method (Questionnaire on the course by the end of the semester) Effectiveness of Students' assessment Head of the department Student achievement is verified by the head of the department. Quality of Learning resources Instructor Student ü Course Report. ü Course Evaluation Survey (CES) Achievement of Course Learning Outcomes Instructor Students ü Direct Method (Rubrics) ü Indirect Method (Questionnaire on the course by the end of the semester) ü Course Evaluation Survey (CES) Effectiveness of Assessment Results Instructor ü Course Report ü Grade Report Analysis ü Course Evaluation Survey (Indirect) Assessors(Students, Faculty, Program Leaders, Peer Reviewer, Others (specify) Assessment Methods(Direct, Indirect)