Course Title: Histopathological Techniques Course Code: CLS 248 Program: Clinical Laboratory Sciences Department: Clinical Laboratory Sciences College: Applied Medical Sciences Institution: Taibah University Version: 2 Last Revision Date: 07/01/1438 AH A. General information about the course: 1. Course Identification 1. Credit hours: ( 3 ) 2 T + 1 P 2. Course type A. ? University ?College ? Department ? Track ?Others B. ? Required ?Elective 3. Level/year at which this course is offered: (Level 4/ Year 2) 4. Course general Description: This course provides students with the theoretical and practical basics of tissue preparation techniques. This including tissue processing, fixation, dehydration, clearing, impregnation, embedding and casting of tissues. The course also gives knowledge on tissue decalcification, sectioning of pathological specimens by various microtomes, staining applying routine pathology stains and special stains, mounting and examination of the slides under the light microscope. In addition, this course would cover frozen sections, cytological preparations, immunohistochemistry and brief knowledge on microscopes in use for pathological studies. 5. Pre-requirements for this course (if any): Histology & Cytology (CLS 235) 6. Co-requisites for this course (if any): None 7. Course Main Objective(s): 1- Provide the students with the knowledge and practical training to deal professionally with the microscopes and other instrument in the histopathology laboratory. 2- Provide the students with the knowledge and practical training to perform tissue preparation techniques. Such as tissue processing which includes fixation, dehydration, clearing, impregnation, embedding and casting of tissues. 3- Provide the students with the knowledge and practical training to perform routine and special stains. 4- Provide the students with the knowledge and practical training to perform frozen section, cytological techniques, immuno-histochemistry. 5- Correlate between the requested analysis and the technique to be used. 2. Teaching mode (mark all that apply) No Mode of Instruction Contact Hours Percentage 1 Traditional classroom/lab sessions 60 100% 2 E-learning 3 Hybrid • Traditional classroom • E-learning 4 Distance learning 3. Contact Hours(based on the academic semester) No Activity Contact Hours 86. Lectures 30 87. Laboratory/Studio 30 88. Field 0 89. Tutorial 90. Others (specify) 0 Total 60 B. Course Learning Outcomes (CLOs), Teaching Strategies and Assessment Methods Code Course Learning Outcomes Code of CLOs aligned with program Teaching Strategies Assessment Methods 1.0 Knowledge and understanding 1.1 Acquire the required level of knowledge and skills on histopathology instrumentation for obtaining a good histology section for pathological examination (e.g. tissue processor, microtomes, microscopes, etc.) K2 Lectures & lab practice Quizzes Midterm exam Final Exam Lab exams Code Course Learning Outcomes Code of CLOs aligned with program Teaching Strategies Assessment Methods 1.2 Explain the routine and special staining procedures and the theory behind them, including histological, cytological, and immune-histochemical and enzyme histochemical stains. K3 Lectures & lab practice -Quizzes, -Laboratory -continuous assessment -Practical exam 2.0 Skills 2.1 Compare between the requested analysis, the parts of tissue specimens to be examined and the technique to be used. S2 Lectures & lab practice Lab continuous assessment Practical exam 2.2 Apply the scientific knowledge and technical procedures in-use for routine paraffin and frozen sections as also in cytology preparations. S1 Lectures & lab practice -Quizzes, -Laboratory -continuous assessment - Practical exam 2.3 Predict the different tissue artifacts and troubleshoot to avoid them. S3 Lectures & lab practice Laboratory continuous assessment Practical exam 3.0 Values, autonomy, and responsibility 3.1 Show a proper equipment handling and resource usage for the department instruments and chemicals in a responsible manner, and always keeping them intact and clean. V1 Lab practice sessions Laboratory continuous assessment C. Course Content No List of Topics Contact Hours 21. Introduction to Histopathological techniques 3 22. Microscopy 3 3. Tissue fixation 4 4. Bone decalcification 3 5. Grossing and sample handling 3 6. Tissue processing of pathological specimens 4 7. Microtomes and Microtomy 4 8. Staining (routine and special stains) 9 9. Frozen and related sections 4 10. Cytological preparations and procedures 4 11 Immunohistochemistry, Enzyme-histochemistry and immunofluorescence techniques 7 13. Artifacts in pathological sections 4 14. Quality Control in Histopathology Laboratory 4 15. Advanced techniques in histopathology (TMAs, Digital pathology, FISH) 4 Total 60 D. Students Assessment Activities No Assessment Activities * Assessment timing (in week no) Percentage of Total Assessment Score 52. Assignment Week 12 5% 53. Mid-Term Exam Week 8-9 15% 54. Continuous Assessment: e.g. Quizzes, Internetsearches, HomeWorks, Exercises, Class activity, Scratch Cards, Presentations, Group work All over the term 5% 55. Lab reports Week 14 5% 56. Practical exam Week 15-16 30% 57. Final Theoretical Exam Week 17-18 40% *Assessment Activities (i.e., Written test, oral test, oral presentation, group project, essay, etc.). E. Learning Resources and Facilities 1. References and Learning Resources Essential References Bancroft's Theory and Practice of Histological Techniques. S. Kim Suvarna, Christopher Layton, and John D. Bancroft 8th edition 2019 Supportive References - Basic and Advanced Laboratory Techniques in Histopathology. Pranab Dey · 2018 - Immunohistochemistry: Basics and Methods Igor B. Buchwalow, Werner B?cker · 2010 Electronic Materials About Histotechnology - National Society for Histotechnology (nsh.org) Other Learning Materials None 2. Required Facilities and equipment Items Resources facilities (Classrooms, laboratories, exhibition rooms, simulation rooms, etc.) Classroom Teaching laboratory Technology equipment (projector, smart board, software) Projector Internet Other equipment (depending on the nature of the specialty) Light microscopes (at least 20) Dissection tool sets Tissue processor Plastic cassette Embedding machine with cold plate Embedding moulds Microtomes Disposable blades for microtome Floating water bath Glass slides Slides coverslips Hot plates Cytology swab Staining jars and staining consumables (chemicals) F. Assessment of Course Quality Assessment Areas/Issues Assessor Assessment Methods Effectiveness of teaching Instructor ü Direct Method (Rubrics) ü Indirect Method (Questionnaire on the course by the end of the semester) Effectiveness of Students' assessment Head of the department Student achievement is verified by the head of the department. Quality of Learning resources Instructor Student ü Course Report. Assessment Areas/Issues Assessor Assessment Methods ü Course Evaluation Survey (CES) Achievement of Course Learning Outcomes Instructor Students ü Direct Method (Rubrics) ü Indirect Method (Questionnaire on the course by the end of the semester) ü Course Evaluation Survey (CES) Effectiveness of Assessment Results Instructor ü Course Report ü Grade Report Analysis ü Course Evaluation Survey (Indirect) Assessors(Students, Faculty, Program Leaders, Peer Reviewer, Others (specify) Assessment Methods(Direct, Indirect)