Course Title: Body fluid analysis and Toxicology Course Code: CLS 479 Program: Clinical Laboratory Sciences Department: Clinical Laboratory Sciences College: Applied Medical Sciences Institution: Taibah University Version: 2 Last Revision Date: 6/1/1438 AH A. General information about the course: 1. Course Identification 1. Credit hours: (3) 2 T + 1 P 2. Course type A. ?University ?College ? Department ?Track ?Others B. ? Required ?Elective 3. Level/year at which this course is offered: (level 7/year 4) 4. Course general Description: By the end of this course, students will enhance their understanding of body fluid and urinalysis laboratory testing principles and procedures. This course equips students with the foundational knowledge for conducting urine and body fluid analysis. Topics covered include renal system anatomy and physiology, urine formation, physical and chemical urine analysis, microscopic examination of urinary sediment, urinary and metabolic diseases, and correlating abnormal findings with disease states. Additionally, the course explores the analysis of cerebrospinal fluid, semen, synovial fluid, serous fluid, sweat fluid, amniotic fluid, and feces, emphasizing their clinical significance. 5. Pre-requirements for this course (if any): Clinical biochemistry (2) CLS 368 6. Co-requisites for this course (if any): None 7. Course Main Objective(s): 1. List the main types of body fluids. 2. Understand the clinically important biochemical & physiological related aspects. 3. Identify the required methods of analysis. 4. Perform physical, chemical, and microscopic analysis of urine specimens. 5. Perform physical, chemical, and microscopic examination of CSF, semen, synovial fluid, serous fluid, sweat fluid, amniotic fluid, and fecal analysis using established laboratory protocol. 6. Define therapeutic drug level and drug safety and efficacy. 7. Analyze and interpret experimental findings in Toxicology. 8. Understand clinical work in toxicology lab and committed to strong ethical and professional values. 9. Interpret the various results of analysis. 10. Evaluate quality control values to determine analytical errors and implement corrective action. 2. Teaching mode (mark all that apply) No Mode of Instruction Contact Hours Percentage 1 Traditional classroom 60 100% 2 E-learning 3 Hybrid • Traditional classroom • E-learning 4 Distance learning 3. Contact Hours(based on the academic semester) No Activity Contact Hours 167. Lectures 30 168. Laboratory/Studio 30 169. Field 170. Tutorial 171. Others (specify) Total 60 B. Course Learning Outcomes (CLOs), Teaching Strategies and Assessment Methods Code Course Learning Outcomes Code of CLOs aligned with program Teaching Strategies Assessment Methods 1.0 Knowledge and understanding 1.1 Describe basic biochemical & physiological knowledge related to human body fluids and monitoring of drugs with thier toxicity. K1 • Lectures are designed to develop the directions for extra reading by the students on the topics. • Guiding students to using references to extract information's. Theoretical exams would contain: • Objective questions (Factual recall, illustrative and/or extended MCQs & fill in blanks). 1.2 Recognize abnormal composition of body fluids and use it for diagnose of deterioration, also recognize drug poisons K2 Code Course Learning Outcomes Code of CLOs aligned with program Teaching Strategies Assessment Methods action, preventions, and diagnosis. • Discussion of the previous topic. • Interpret of the lecture's outcome every week. • Report and comment on cases study. • Short essay questions • Long essay questions 1.3 Correlate between different tests to distinguish causes of abnormality in fluid composition. K3 2.0 Skills 2.1 Perform professional chemical and microscopic examinations of fluids and drugs with precision. Efficiently select necessary lab tests for fluid analysis and toxicology. S1 • Discussions during formal lectures and /or seminars. • Discuss the theory pre and post the practical work. • interpret the findings of laboratory investigations Follow up within the practical sessions: • Objective Structured Practical Examination (OSPE). • Problem solving questions 2.2 Interpret various disorders that could be diagnosed by analysis of these fluids. S3 3.0 Values, autonomy, and responsibility 3.1 Generate teamwork activities, sharing of personal ideas, and making group discussion. V1 • Group discussion in theory and practical. • Group and individual internet search and analysis for selected topics • Class and lab discussion. • Instructor evaluation and support activity participation in lectures and practical. • Regularly reports. • Assignments and Homework C. Course Content No List of Topics Contact Hours 65. Introduction to urine analysis: Formation & composition of urine 4 2. Examination of urine: physical, chemical & Microscopy 4 3. Interpretation of normal & abnormal urine reports 4 4. Special urinary tests: their clinical significance 4 5. Special urinary tests: their clinical significance and urinary calculi 4 6. Introduction to CSF analysis 4 7. Introduction to semen Analysis 4 8. Examination of serous fluids 4 9. Examination of Sweat fluid, amniotic fluid, synovial fluid & feces 4 10. Introduction to Therapeutic Drug Monitoring 4 11. Pharmacokinetics & Pharmacogenomics 4 12. Introduction to Toxicology: Exposure to toxins & Routes of exposure 4 13. Dose-response relationship 4 14. Analysis of toxic agents & toxicology of specific agents 4 15. Toxicology of therapeutic drugs & drug abuse 4 Total 60 D. Students Assessment Activities No Assessment Activities * Assessment timing (in week no) Percentage of Total Assessment Score 117. Continuous assessment All over course 5% 118. Midterm examination (written) Week 8-9 15% 119. Assignment submission Week 12 5% 120. Lab report Week 14 5% 5. Practical exam Week 15-16 30% 6. Final written examination Week 17-18 40% E. Learning Resources and Facilities 1. References and Learning Resources Essential References 1. Nancy A. Brunzel. Fundamentals of Urine and Body Fluid Analysis, 4th ed. Saunders, an imprint of Elsevier Inc. 2018. ISBN: 978-1-4377-0989-6. 2. Ging, P. M., & R. O. (2009). The biochemistry of body fluids. (4th ed.). ACBI Scientific Committee Guidelines. 3. Michael L. Bishop et.al.(2014) Clinical chemistry principles, procedures, correlations. Lippincott. Supportive References 1. Varley H. (2002). Practical clinical biochemistry; 4th edition. Arnold. 2. King-Strasinger S, Schaub Di Lorenzo M. Urinalysis and Body Fluids, 5th ed. Philadelphia: FA Davis, 2008. 3. Lillian A. Mundt and Kristy Shanahan, EdD, Graff ’s Textbook of Routine Urinalysis and Body Fluids, 2nd ed. LIPPINCOTT WILLIAMS & WILKINS, a WOLTERS KLUWER business 2011. ISBN 978-1-58255- 875-2. Ringsrud K M and Linne J J. Urinalysis and Body Fluids A Color Text and Atlas, 1st ed. St. Louis, Mosby, 1995. ISBN: 0-8016- 7043-8. Electronic Materials 1. Youtube. https://www.youtube.com/ 2. Google images. https://www.google.com.sa/ 3. Taibah University Library e-Book database. https://www.taibahu.edu.sa/Pages/AR/Sector/SectorPage.aspx?ID=4 7 4.http://www.acgme.org/acWebsite/RRC_300/300_prIndex.asp Other Learning Materials 1. Selected films/video clips. 2. Internet resources/. 3. Urinalysis: An Educational Program (cat. #QY 185 U76) a. “The Urinary System”. b. “The Urine Specimen”. c. “Physical Examination of the Urine”. d. “Chemical Analysis of the Urine”. e. “Microscopic Examination of the Urine”. 2. Required Facilities and equipment Items Resources facilities (Classrooms, laboratories, exhibition rooms, simulation rooms, etc.) Lecture room. Clinical biochemistry Lab (with microscopes). Toxicology lab Technology equipment (projector, smart board, software) Data show. Laptop. Other equipment (depending on the nature of the specialty) Internet accessibility. F. Assessment of Course Quality Assessment Areas/Issues Assessor Assessment Methods Effectiveness of teaching Instructor ü Direct Method (Rubrics) ü Indirect Method (Questionnaire on the course by the end of the semester) Effectiveness of Students' assessment Head of the department Student achievement is verified by the head of the department. Quality of Learning resources Instructor Student ü Course Report. ü Course Evaluation Survey (CES) Achievement of Course Learning Outcomes Instructor Students ü Direct Method (Rubrics) ü Indirect Method (Questionnaire on the course by the end of the semester) ü Course Evaluation Survey (CES) Effectiveness of Assessment Results Instructor ü Course Report ü Grade Report Analysis ü Course Evaluation Survey (Indirect) Assessors(Students, Faculty, Program Leaders, Peer Reviewer, Others (specify) Assessment Methods(Direct, Indirect)