Course Title: Molecular Biology Course Code: CLS 396 Program: Clinical Laboratory Sciences Department: Clinical Laboratory Sciences College: Applied Medical Sciences Institution: Taibah University Version: 2 Last Revision Date: 20/01/1438 AH A. General information about the course: 1. Course Identification 1. Credit hours: (3) 2 T + 1 P 2. Course type A. ?University ?College ? Department ?Track ?Others B. ? Required ?Elective 3. Level/year at which this course is offered: (6th level/3rd year) 4. Course general Description: This course describes the basic concepts and principles of molecular biology from nucleic acid and protein structure to the most recent and state of art techniques for detection and analysis of biomolecules, DNA, RNA and protein. Gene structure, function and expression are stated. The molecular cellular mechanisms controlling DNA replication during cell division and gene expression is well explained. Most relevant molecular methods and techniques that are used to extract and analyze the biomolecules from clinical samples are listed and complemented by further illustration and hand on method practice. Applications of both PCR and DNA sequencing in medical clinical practice to detect and confirm gene mutations are well described. A most advanced and up to date molecular biology techniques that are being introduced to molecular diagnostic labs are summarized. Wealth of bioinformation related to structures, functions and mutations of biomolecules are being stored in different but connected and accessible databases on the net facilitating emerging of a new field of science termed bioinformatics. 5. Pre-requirements for this course (if any): Molecular & Medical Genetics (CLS 393) 6. Co-requisites for this course (if any): NA 7. Course Main Objective(s): 1) Define molecular biology and its relation to genetics and biochemistry. 2) Recognize structures of nucleic acids. 3) Explain the gene molecular structure, function, mutation, and expression. 4) Describe the central dogma of molecular biology and the major stages in DNA replication, mRNA transcription and translation. 5) Identify recombinant DNA technology and its applications. 6) Describe different PCR types and their applications. 7) Illustrate different molecular techniques for analysis of DNA, RNA, and proteins biomolecules. 8) Define bioinformatics and state different biomolecule databases. 9) Outline the major molecular diagnostic techniques in clinical labs. 2. Teaching mode (mark all that apply) No Mode of Instruction Contact Hours Percentage 1 Traditional classroom 60 100% 2 E-learning 3 Hybrid • Traditional classroom • E-learning 4 Distance learning 3. Contact Hours(based on the academic semester) No Activity Contact Hours 142. Lectures 30 143. Laboratory/Studio 30 144. Field 145. Tutorial 146. Others (specify) Total 60 B. Course Learning Outcomes (CLOs), Teaching Strategies and Assessment Methods Code Course Learning Outcomes Code of CLOs aligned with program Teaching Strategies Assessment Methods 1.0 Knowledge and understanding 1.1 Describe molecular structure, function of nucleic acids and genes, elaborating K1 • Formal and interactive lectures • Homework and problem sets • Exams • Quizzes • Assignments • Student evaluation Code Course Learning Outcomes Code of CLOs aligned with program Teaching Strategies Assessment Methods on the processes of DNA replication, transcription, and translation along with the various types of mutations and their consequences on gene expression. 1.2 Explain the principles and applications of various molecular techniques. K3 • Formal and interactive lectures • Homework and problem sets • Exams • Quizzes • Assignments • Student evaluation 2.0 Skills 2.1 Demonstrate a thorough understanding of laboratory safety protocols and procedures relevant to molecular biology techniques. S1 • Lectures • Practical sessions • Exams •Quizzes • Assignments and reports • Student evaluation 2.2 Master proper handling of molecular biology instruments, accurate analysis, and interpretation of experimental data, while also applying fundamental bioinformatics skills such as database search, sequence S2 • Lectures • Practical sessions • PBL • Small Internet based project • Exams •Quizzes • Assignments and reports • Student evaluation Code Course Learning Outcomes Code of CLOs aligned with program Teaching Strategies Assessment Methods analysis, and primer design 3.0 Values, autonomy, and responsibility 3.1 Managing molecular laboratory’s procedures, instruments, and results independently. V2 • Lectures • Practical sessions • Exams • Lab reports 3.2 Demonstrate good ethical behavior and communication skills for collaborative work with team members. V3 • Lectures • Practical sessions • Group discussions • Student evaluation in class and lab • Evaluation of group activities • Evaluation of presentations C. Course Content No List of Topics Contact Hours Theory 54. Introduction to molecular biology 2 2. Gene and genome 2 3. Biomolecule structures and DNA mutations 2 4. DNA replication 2 5. Transcription 2 6. RNA processing and post-transcriptional gene regulation 2 7. Translation 2 8. Bioinformatics 2 10. Recombinant DNA technology 2 11. Polymerase chain reaction 2 12. Molecular techniques I 2 13. Molecular techniques II 2 14. Molecular techniques III 4 Total 30 Practical 1. Molecular Biology lab safety 2 55. Genomic DNA extraction 2 3. Nucleic acid quantification 2 4. Gel electrophoresis 2 5. PCR 4 6. PCR-RFLP 4 7. qRT-PCR 2 8. RNA extraction and RT-PCR 2 9. DNA sequencing 2 10. Bioinformatics 4 11. Western blot 2 12. Revision 2 Total 30 D. Students Assessment Activities No Assessment Activities * Assessment timing (in week no) Percentage of Total Assessment Score 95. Assignments Week 12 5% 96. Mid-Term Exam Week 8-9 15% 97. Lab reports Week 14 5% 98. Continuous Assessment (Quizzes, case studies, presentation, Journal club, etc..) All over the course 5% 4. Practical Exam Week 15-16 30% 5. Final written Exam Week 17-18 40% *Assessment Activities (i.e., Written test, oral test, oral presentation, group project, essay, etc.). E. Learning Resources and Facilities 1. References and Learning Resources Essential References 1) Weaver, Robert F. Molecular Biology, 5th ed. MGrawHill education, 2015. 2) Watson, James et al. Molecular biology of the gene, 7th ed. Supportive References 1) Allison, Lizabeth A. Fundamental Molecular Biology, 3rd.John Wiley & sons, 2021. 2) Lewin, Benjamin. Essential Genes. 4th ed. Benjamin Cummings, 2021. Electronic Materials 1) SlideShare (http://www.slideshare.net ) 2) Learn science at Nature (http://www.nature.com/scitable) 3) Journal of molecular biology (http://www.sciencedirect.com/science/journal/00222836) 4) Coursera (https://www.coursera.org) 5) EDx (https://www.edx.org) Other Learning Materials 1) Access DNA and protein data bases. 2) Mendel genetic disease database. 2. Required Facilities and equipment Items Resources facilities (Classrooms, laboratories, exhibition rooms, simulation rooms, etc.) Classrooms laboratories Technology equipment (projector, smart board, software) Projector Laptop Data show Other equipment (depending on the nature of the specialty) Centrifuges PCR instruments Gel electrophoresis apparatus UV transilluminator Nanodrop Pipettes Water bath Vortex F. Assessment of Course Quality Assessment Areas/Issues Assessor Assessment Methods Effectiveness of teaching Instructor ü Direct Method (Rubrics) ü Indirect Method (Questionnaire on the course by the end of the semester) Effectiveness of Students' assessment Head of the department Student achievement is verified by the head of the department. Quality of Learning resources Instructor Student ü Course Report. ü Course Evaluation Survey (CES) Achievement of Course Learning Outcomes Instructor Students ü Direct Method (Rubrics) ü Indirect Method (Questionnaire on the course by the end of the semester) ü Course Evaluation Survey (CES) Assessment Areas/Issues Assessor Assessment Methods Effectiveness of Assessment Results Instructor ü Course Report ü Grade Report Analysis ü Course Evaluation Survey (Indirect) Assessors(Students, Faculty, Program Leaders, Peer Reviewer, Others (specify) Assessment Methods(Direct, Indirect)